![]() ![]() Ma Q, Lu AYH (2011) Pharmacogenetics, pharmacogenomics, and individualized medicine. Hauser AS, Chavali S, Masuho I et al (2018) Pharmacogenomics of GPCR drug targets. ![]() Hoofnagle JH, Bjornsson ES (2019) Drug-induced liver injury-types and phenotypes. Nat Rev Gastroenterol Hepatol 16(1):57–73. Wong MCS, Huang JLW, George J et al (2019) The changing epidemiology of liver diseases in the Asia-Pacific region. Navarro VJ, Khan I, Bjoernsson E et al (2017) Liver injury from herbal and dietary supplements. Navarro VJ, Barnhart H, Bonkovsky HL et al (2014) Liver injury from herbals and dietary supplements in the US drug-induced liver injury network. Ren X, Xin LT, Zhang MQ et al (2019) Hepatoprotective effects of a traditional Chinese medicine formula against carbon tetrachloride-induced hepatotoxicity in vivo and in vitro. Zhao P, Wang C, Liu W et al (2014) Acute liver failure associated with traditional Chinese medicine: report of 30 cases from seven tertiary hospitals in China*. The results combined of this project are promising for future research, especially regarding the use of liver-derived organoids for recellularization.Asrani SK, Devarbhavi H, Eaton J et al (2019) Burden of liver diseases in the world. The qPCR data showed variability between the different organoid lines and between the different phases of the organoid culture. The liver-derived organoids were successfully cultured and expanded in spinnerflasks, and were found engrafted and alive after 10 days in the scaffold. The HepG2 cell line validated the set up and recellularization with the HA, the cells engrafted throughout the scaffold and showed viability and signs of proliferation. The experiments were analyzed by histological and immunochemical staining and by qPCR. A setback in the project was the proneness to infections in the Harvard Apparatus (HA), which shortened the duration of experiments and influenced the results. In the bioreactor, the Harvard Apparatus (Hugo Sachs Elektronik), decellularized porcine liver segments were infused with a HepG2 cell line and liver-derived organoids in seperate experiments. This project shows that it is possible to use a perfusion-based bioreactor for repopulating a porcine liver scaffold with liver-derived organoids. However, there is no optimal method yet to recellularize such a decellularized liver scaffold. This ECM provides the biophysical and biochemical cues needed for cells to adhere, proliferate and differentiate. Luckily, the decellularization of an organ creates a scaffold that consists of the extracellular matrix (ECM) with important growth factors, bifunctional molecules such a fibronectin and multiple collagen types. Engineering organs with a complex structure and large vascular network, such as the liver, remain a challenge. Over the last decade new methods are explored in the field of tissue engineering to minimize the donor organ shortage. Vermeulen, Annewiet (TU Delft Mechanical, Maritime and Materials Engineering)īiomedical Engineering | Biomaterials and Tissue Biomechanics Repopulating a decellularized liver scaffold with liver-derived organoids in a perfusion-based bioreactor ![]()
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